RBX1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499278
• Category: Antibodies
• Description: RBX1 Recombinant Rabbit Monoclonal Antibody
• Target: RBX1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human RBX1 (60-108/108aa)
• UniProt ID: P62877
• MW: 12 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100
• Antibody Type: Primary Antibody
• Clone Number: R2D3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RBX1_HUMAN; E3 ubiquitin-protein ligase RBX1; EC:2
• Research Area: Cell Cycle, Cell Signaling, E3 ubiquitin ligases,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: HepG2, 3: HEK-293, 4: NIH/3T3, 5: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 12 kDa, Observed MW: 12 kDa.

Tissue: Human bladder cancer, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue).

Tissue: Rat stomach, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499278.