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| Catalog Number | orb1173586 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretin(SCT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretin(SCT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretin(SCT) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rat |
| Concentration | 1000 pg/mL |
| UniProt ID | P11384 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCT in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.78 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | Sct , Secretin |
| Note | For research use only |
Rat Secretin ELISA Kit [orb410790]
Rat
3.12 pg/mL-200 pg/mL
0.78 pg/mL
10 x 96 T, 48 T, 5 x 96 T, 96 T, 24 T- Item 1 of 1
- Item 1 of 1
