Rat Secretagogin (SCGN) ELISA Kit

• Catalog Number: orb780207
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretagogin(SCGN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretagogin(SCGN).
• Reactivity: Rat
• Concentration: 5000 pg/mL
• UniProt ID: Q6R556
• Tested applications: ELISA
• Application notes: standard: 5000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 23.9 pg/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: SECRET ELISA Kit, SEGN ELISA Kit, CALBL ELISA Kit,
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.