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Rat Galanin (GAL) ELISA Kit

Catalog Number: orb779494

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb779494
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Galanin(GAL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galanin(GAL).
ReactivityRat
Concentration1000 pg/mL
UniProt IDP10683
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GAL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAL in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity4.94 pg/mL
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesGALN ELISA Kit, GLNN ELISA Kit, GMAP ELISA Kit, Ga
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NoteFor research use only
Rat Galanin (GAL) ELISA Kit

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