Rat Fibrinopeptide B (FPB) ELISA Kit

• Catalog Number: orb780290
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fibrinopeptide B(FPB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibrinopeptide B(FPB).
• Reactivity: Rat
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FPB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FPB in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.46 ng/mL
• Sample Types: Plasma.
• Alternative names: FP-B ELISA Kit
• Research Area: Hematology, Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.