Rat 6-keto-PGF1 Alpha (6-K-PGF1α) ELISA Kit

• Catalog Number: orb782160
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1 alpha protein.
• Reactivity: Rat
• Concentration: 500 pg/mL
• Tested applications: ELISA
• Application notes: standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 6ketoPGF1α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 7.8 pg/ml - 500 pg/ml
• Sensitivity: 1.4 pg/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: 6-keto-PGF1 alpha ELISA Kit, 6ketoPGF1α ELISA Kit
• Research Area: Neuroscience
• Note: For research use only
• Expiration Date: Please enquire.