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rApi g 1 (Apium graveolens 1.0101)
Catalog Number: orb1088535
| Catalog Number | orb1088535 |
|---|---|
| Category | Proteins |
| Description | Recombinant protein rApi g 1 is expressed in Escherichia coli. DNA sequence encoding 167 AAs was fused with His-tag at the C-terminus. A calculated molecular mass of recombinant protein is 17,9 kDa. |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | HEPES buffered saline (HBS), pH 7.4 |
| Purity | Purified by sequential steps of ion exchange and affinity chromatography. |
| Purification | Purified by sequential steps of ion exchange and affinity chromatography. |
| UniProt ID | P49372 |
| Tested applications | ELISA, FC |
| Storage | Store at -20°C to -80°C. |
| Note | For research use only |
Recombinant allergen rApi g 1 purity verification. 5 µg of rApi g 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
Flow cytometry dot-plot staining pattern of rApi g 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
