Raf-B (phospho Ser446) rabbit pAb

• Catalog Number: orb770155
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Raf-B (phospho-Ser446)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser446. AA range:411-460
• MW: 110
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-BRAF antibody, anti-BRAF1 antibody, anti-RAFB
• Note: For research use only

Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000.

Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-RAF (Phospho-Ser446) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is blocked with the phospho peptide.