Rabbit anti-Sp1 Antibody

• Catalog Number: orb1530643
• Category: Antibodies
• Description: Sp1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 735 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Sp1
• UniProt ID: P08047
• NCBI: XP_028606.4
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: specificity protein 1; transcription factor Sp1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SP1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, MCF-7, and CCRF-CEM cells. Antibody: Affinity purified rabbit anti-SP1 antibody at 0.1 µg/ml.

Detection of mouse SP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human SP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human SP1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-SP1 antibody.

Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing.