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Rabbit anti-Rictor Antibody
Catalog Number: orb1523195
| Catalog Number | orb1523195 |
|---|---|
| Category | Antibodies |
| Description | Rictor Antibody |
| Target | Rictor |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 1000 µg/ml |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Immunogen | Between 1 and 50 |
| UniProt ID | Q6R327 |
| Tested applications | IHC, IP |
| Dilution range | WB - Not recommended. A304-329A has not performed satisfactorily when used for WB of Rictor in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Rictor.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | hAVO3; TORC2-specific protein AVO3; rapamycin-inse Read more... |
| Note | For research use only |
| NCBI | NP_689969.2 |
Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Rictor antibody orb1523195 used for IP at 6 µg per reaction.
Detection of human Rictor by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. orb1523195) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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