Rabbit anti-RACK1 Antibody

• Catalog Number: orb1526438
• Category: Antibodies
• Description: RACK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RACK1
• UniProt ID: P63244
• NCBI: NP_006089.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: small ribosomal subunit protein RACK1; lung cancer
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse RACK1 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RACK1 antibody (orb1526438) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RACK1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RACK1 antibody (orb1526438) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RACK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RACK1 antibody (orb1526438) used for IP at 6 µg per reaction. RACK1 was also immunoprecipitated by a previous lot of this antibody (orb1526438) and a second antibody against a different epitope of RACK1.