Rabbit anti-Phospho MCM2 (S53) Recombinant Monoclonal Antibody

• Catalog Number: orb1519726
• Category: Antibodies
• Description: MCM2, Phospho (S53) Antibody
• Target: MCM2, Phospho (S53)
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 250 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Surrounding Serine 53
• UniProt ID: P49736
• Tested applications: ICC, IHC, WB
• Dilution range: WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR108H
• Storage: 2 - 8°C
• Alternative names: D3S3194; MITOTIN; minichromosome maintenance prote
• Note: For research use only
• NCBI: NP_004517.2
• Expiration Date: 12 months from date of receipt.

Detection of mouse Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519726) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal.

Detection of human Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519726) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.

Detection of human Phospho-MCM2 (S53) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MCM2 antibody used for IP. Immunoprecipitates where either treated with phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho-MCM2 (S53) , orb1519726 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Total MCM2.