Rabbit anti-NKG2A Recombinant Monoclonal Antibody

• Catalog Number: orb1806440
• Category: Antibodies
• Description: Rabbit anti-NKG2A Recombinant Monoclonal Antibody
• Target: NKG2A
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Immunogen: Between 1 and 50
• UniProt ID: P26715
• Tested applications: IHC, WB
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR290L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B typ
• Note: For research use only

Detection of human NKG2A by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, NK-92, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NKG2A recombinant monoclonal antibody (orb1806440) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human NKG2A by immunohistochemistry. Sample: FFPE section of Hodgkin Lympnoma. Antibody: Rabbit anti-NKG2A recombinant monoclonal antibody (orb1806440). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human NKG2A by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-NKG2A recombinant monoclonal antibody (orb1806440). Secondary: HRP-conjugated goat anti-rabbit IgG.