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Catalog Number | orb1526230 |
---|---|
Category | Antibodies |
Description | MTMR15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MTMR15 |
UniProt ID | Q9Y2M0 |
NCBI | NP_055782.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | MTMR15; myotubularin-related protein 15; KMIN; KIA Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human MTMR15 by western blot. Samples: Whole cell lysate (10 µg) from GaMG and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTMR15 antibody (orb1526230) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human MTMR15 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTMR15 antibody (orb1526230) used for IP at 6 µg per reaction.
Detection of human MTMR15 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MTMR15 (orb1526230) used at a dilution of 1: 200 (1µg/ml). Detection: DAB