Rabbit anti-IL-12B Recombinant Monoclonal Antibody

• Catalog Number: orb2281284
• Category: Antibodies
• Description: Rabbit anti-IL-12B Recombinant Monoclonal Antibody
• Target: IL-12B
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, rAlbumin-Free
• Immunogen: residues 23-328
• UniProt ID: P29460
• Tested applications: IP, WB
• Dilution range: WB: 1:1,000, IP: 6 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR322M
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: interleukin 12, p40; interleukin 12B (natural kill
• Note: For research use only

Detection of human IL-12B by western blot. Samples: Whole cell lysate (50 µg) from 293F cells untransfected (-) or overexpressing IL-12A, IL-12B, IL-12A and IL-12B, or IL-23A and IL-12B. Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second

Detection of human IL-12B by western blot. Samples: Human recombinant IL-12B protein (0.01 µg). Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second