Rabbit anti-BRD2 Antibody

• Catalog Number: orb1526367
• Category: Antibodies
• Description: BRD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 700 and 750
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:20 to 1:100. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD2
• UniProt ID: P25440
• NCBI: NP_005095.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: D6S113E; RING3; really interesting new gene 3 prot
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse BRD2 by western blot. Samples: Whole cell lysate (50 µg) from RenCa and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526367) used for WB at 0.04 µg/ml.

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody.

Detection of human BRD2 by immunohistochemistry.Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (orb1526367) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human BRD2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (orb1526367)