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Catalog Number | orb1530279 |
---|---|
Category | Antibodies |
Description | Bcl11b Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 500 and 550 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:5,000 - 1:15,000; IP - 1 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Bcl11b |
UniProt ID | Q9C0K0 |
NCBI | NP_612808.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | hRIT1-alpha; ZNF856B; zinc finger protein hRit1 al Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Bcl11b by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-Bcl11b antibody orb1530279 at 0.066 µg/ml.
Detection of human Bcl11b by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1, 000 (1µg/ml).
Detection of human Bcl11b by WB and IP.Samples: Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody used at 0.1 µg/ml (A) and 0.5 µg/ml (B) for WB and at 1 and 10 µg/mg lysate for IP (B).