PSD95 Antibody: Biotin

• Catalog Number: orb147007
• Category: Antibodies
• Description: Mouse monoclonal to PSD95 (Biotin). Postsynaptic Density protein 95 (PSD95), also known as Postsynaptic Density protein 95 (PSD95), also known as...
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6G6
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Recombinant rat PSD-95
• Concentration: 1 mg/ml
• Dilution range: WB (1:250), IHC (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 75kDa
• Target: PSD95
• Entrez: 29495
• UniProt ID: P31016
• NCBI: NP_062567.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: PSD 95 antibody, PSD-95 antibody, DLG4 antibody, S
• Note: For research use only
• Application notes: 1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.

Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.