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| Catalog Number | orb125220 |
|---|---|
| Category | Antibodies |
| Description | Goat Polyclonal to PRODH. NP_057419.4; NP_001182155.1, PMID: 18163391 and GeneID: 5625. |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Dilution range | P-ELISA:1:16000, WB:0.3-1µg/ml |
| Conjugation | Unconjugated |
| MW | 68.0; 56.2 |
| Target | PRODH (aa112-134) |
| Entrez | 5625 |
| Protein Sequence | EDQESIQPLLRHYR |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti PRODH antibody, anti proline dehydrogenase (o Read more... |
| Note | For research use only |
| Application notes | WB: Approx75+55kDa bands observed in Human Brain (Cerebellum and Substantia nigra) lysates (calculated MW of 68.0kDakDa according to NP_057419.4 and of 56.2kDa according to NP_001182155.1). Recommended concentration: 0.3-1µg/ml. |
| Expiration Date | 12 months from date of receipt. |
Zareba, Ilona et al. Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model Cell Physiol Biochem, 43, 670-684 (2017)
0.3 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
