Plant Abscisic Acid (ABA) ELISA Kit

• Catalog Number: orb782357
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Abscisic Acid(ABA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Abscisic Acid(ABA).
• Reactivity: Plant
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant ABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant ABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant ABA in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.53 ng/mL
• Sample Types: plant tissues
• Alternative names: AB-A ELISA Kit, abscisin II ELISA Kit, Dormin ELIS
• Research Area: Metabolism Research
• Note: For research use only