PKM2 Antibody (C-term C474)

• Catalog Number: orb1929508
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-489 amino acids from the C-terminal region of human PKM2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Rabbit
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P14618
• MW: 57937 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB15339
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Research Area: Cancer Biology, Cardiovascular Research, Metabolis
• Note: For research use only
• NCBI: NP_001193726.1, NP_001193727.1, NP_002645.3, NP_872270.1, NP_872271.1, NP_001193725.1, NP_001193728.1

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PKM2 antibody (C-term C474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-PKM2 Antibody (C-term C474) in CEM cell line lysates (35 ug/lane). PKM2 (arrow) was detected using the purified Pab.