Phospho-Smad3 (Ser425) Mouse Monoclonal Antibody

• Catalog Number: orb506114
• Category: Antibodies
• Description: Phospho-Smad3 (Ser425) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E8
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser425 SV(p-S)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: SMAD3
• UniProt ID: P84022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SMAD3(phospho S425); Smad3(phospho Ser425); p-Smad
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Smad3 (Ser425)) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Smad3 (Ser425)) Monoclonal Antibody, Unconjugated (ascites of orb506114) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.