Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559124
• Category: Antibodies
• Description: Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody
• Target: SMAD2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser255 TL(p-S)PV
• UniProt ID: Q15796
• MW: 58 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Clone Number: 8C12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Smad2(S255); Smad2(phospho S255); phospho-Smad2(Se
• Research Area: Cell Biology, Cell Signaling, Cytoplasmic, Intrace
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: (-) HeLa, 2: (+) HeLa + OA (100 mM, 60 min) + CA (100 mM, 60 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 52 kDa, Observed MW: 58 kDa.