Phospho-PSD95 (Tyr236 + Tyr240) Rabbit Polyclonal Antibody

• Catalog Number: orb6813
• Category: Antibodies
• Description: Phospho-PSD95 (Tyr236 + Tyr240) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PSD95 around the phosphorylation site of Tyr236/Tyr240 NT(p-Y)DV
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: DLG4
• UniProt ID: P78352
• RRID: AB_10922831
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PSD95(phospho Tyr236+Tyr240); PSD95(phospho Y236+Y
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-Phospho-PSD95 (Tyr236 + Tyr240) antibody (orb6813), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FTIC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PSD95 (Tyr236 + Tyr240)) Polyclonal Antibody, Unconjugated (orb6813) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PSD95 (Tyr236 + Tyr240)) Polyclonal Antibody, Unconjugated (orb6813) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-P70 S6 beta) Polyclonal Antibody, Unconjugated (orb6813) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.