Phospho-P53 (Ser46) Rabbit Polyclonal Antibody

• Catalog Number: orb6598
• Category: Antibodies
• Description: Phospho-P53 (Ser46) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser46 ML(p-S)PD
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=0.2μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: TP53
• UniProt ID: P04637
• RRID: AB_10935367
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: p53(phospho-Ser46); P53(phospho S46); P53(phospho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: A549 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, MDA-MB-231 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-P53 (Ser46) (orb6598) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-P53 (Ser46) (orb6598) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 55 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-P53 (Ser46) (orb6598) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-P53 (Ser46)) polyclonal Antibody, Unconjugated (orb6598) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-P53 (Ser46)) polyclonal Antibody, Unconjugated (orb6598) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-P53 (Ser46) antibody (orb6598), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-P53 (Ser46) Polyclonal Antibody, Unconjugated (orb6598) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.