Phospho-P53 (Ser376) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559176
• Category: Antibodies
• Description: Phospho-P53 (Ser376) Recombinant Rabbit Monoclonal Antibody
• Target: TP53
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser376 GQ(p-S)TS
• UniProt ID: P04637
• MW: 43 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Clone Number: 7C9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: p53(S376); p53(phospho-Ser376); P53(phospho S376);
• Research Area: Cancer Research, Tumor Suppressors, p53 Signaling
• Note: For research use only

Western blot analysis of Phospho-p53 (S376) on different lysates with Rabbit anti-Phospho-p53 (S376) antibody (orb559176) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate (20 µg/Lane), Lane 2: Raw264.7 cell lysate, Lane 3: NIH/3T3 cell lysate, Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa, Observed band size: 53 kDa, Exposure time: 2 minutes, 10% SDS-PAGE gel.

Western blot analysis of Phospho-p53 (S376) on human skin lysates using anti-Phospho-p53 (S376) antibody at 1/1000 dilution.