Phospho-NFATc2 (Ser330) Rabbit Polyclonal Antibody

• Catalog Number: orb101236
• Category: Antibodies
• Description: Phospho-NFATc2 (Ser330) Rabbit Polyclonal Antibody
• Target: NFATC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser330 DP(p-S)PV
• UniProt ID: Q13469
• MW: 100 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NFATC2(phospho Ser330); NFATC2(phospho S330); AI60
• Research Area: Cancer Research, DNA and RNA Research, Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Minghui Wang a b 1 , Huiying Cai a b 1 , Manman Wang c 1 , Honggang Gao b 1 , Xiaona Zhao b d , Ruixue Rong a b , Jing Zhang b , Zejin Zhang b e , Guoqing Liu a b , Tao Yuan b f , Fangjie Xia b d , Lin Zhu a b , Zhenzhen Yan a b , Xinxin Kong b d , Wei Qin Lonicerin targets ADRA1D and RSPO3 to ameliorate diabetes-induced vascular injury through Ca2+/Calcineurin/NFAT1-dependent anti-EndMT pathway Phytomedicine, (2025)

PubMed:

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-phospho-NFATc2 (Ser330) antibody (orb101236), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-NFATc2 (Ser330)) Polyclonal Antibody, Unconjugated (orb101236) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Human Raji cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-phospho-NFATc2 (Ser330) (orb101236) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 140 kDa.