Phospho-MLKL (Ser345) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb608076
• Category: Antibodies
• Description: Phospho-MLKL (Ser345) Recombinant Rabbit Monoclonal Antibody
• Target: MLKL
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from mouse Mlkl around the phosphorylation site of S345 QN-pS-IS
• UniProt ID: Q9D2Y4
• MW: 54 kDa
• Tested applications: ICC, IP, WB
• Dilution range: WB=1:500-2000, IP=1:10-50, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 7G4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: phospho-MLKL(S345); p-MLKL(S345); MLKL(p-S345); hM
• Research Area: Cancer Research, Kinases, Necroptosis Pathway, SAR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phospho-MLKL (S345) on L929 cell lysates. Lane 1: L929 cells, whole cell lysate, 10 µg/lane. Lane 2: L929 cells were treated with 20 uM Z-VAD for 30 minutes, then added 20 ng/ml mTNF-alpha and 100 nM SM-164 for 4 hours, whole cell lysates, 10 µg/lane.