Phospho-MET (Tyr1003) Rabbit Polyclonal Antibody

• Catalog Number: orb4943
• Category: Antibodies
• Description: Phospho-MET (Tyr1003) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MET around the phosphorylation site of Tyr1003 VD(p-Y)RA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33/123/156 kDa
• Target: MET
• UniProt ID: P08581
• RRID: AB_10927900
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MET_HUMAN; Hepatocyte growth factor receptor; EC:2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: A431 (Human) cell Lysate at 30 ug, Primary: Anti-Phospho-Met (Tyr1003) (orb4943) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kD, Observed band size: 178 kD.

Sample: Lane 1: Rat Liver tissue lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-Phospho-MET (Tyr1003) (orb4943) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33/123/156 kDa, Observed band size: 140 kDa.