Phospho-cdc25C (Ser216) Rabbit Polyclonal Antibody

• Catalog Number: orb5825
• Category: Antibodies
• Description: Phospho-cdc25C (Ser216) Rabbit Polyclonal Antibody
• Target: CDC25C
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216 P(p-S)MP
• UniProt ID: P30307
• RRID: AB_10939115
• MW: 53 kDa
• Tested applications: ELISA
• Dilution range: ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: cdc25C(phospho-Ser216); cdc25C(phospho Ser216); cd
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-cdc25C (Ser216) (orb5825) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.

Sample: Testis (Mouse) lysate at 30 ug, Primary: Anti-Phospho-cdc25C (Ser216) (orb5825) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 53 kD, Observed band size: 49 kD.