Phospho-CDC25A(S124) Antibody

• Catalog Number: orb1931290
• Category: Antibodies
• Description: Phospho-CDC25A(S124) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P30304
• MW: 59087 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-phospho CDC25A S124 Pab is used in Western blot to detect CDC25A in mouse liver tissue lysate

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1 h, using 459085101 (upper) or Beta-actin (lower).

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1 h, using 459088101 (upper) or Beta-actin (lower).