Phospho-C-Myc (Thr358) Rabbit Polyclonal Antibody

• Catalog Number: orb5694
• Category: Antibodies
• Description: Phospho-C-Myc (Thr358) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Equine
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human C-Myc around the phosphorylation site of Thr358 RR(p-T)HN
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: MYC
• UniProt ID: P01106
• RRID: AB_10940868
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Myc(phospho-Thr358); Myc(phospho-T358); p-Myc(Thr3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-C-Myc (Thr358) antibody (orb5694), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: A431 (Human) Lysate at 30 ug U251 (Human) Lysate at 30 ug Huvec (Human) Lysate at 30 ug Raji (Human) Lysate at 30 ug U937 (Human) Lysate at 30 ug Primary: Anti-phospho-C-Myc (Thr358) (orb5694) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD.