PGC Mouse Monoclonal Antibody

• Catalog Number: orb1816836
• Category: Antibodies
• Description: PGC Mouse Monoclonal Antibody
• Target: PGC
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b,κ
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Immunogen: Recombinant human PGC protein (17-388/388aa)
• UniProt ID: P20142
• MW: 40 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 1A4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C;
• Note: For research use only

Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-PGC (orb1816836) at 1/1000 dilution, Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific, Predicted band size: 42 kDa, Observed band size: 39 kDa.

Sample: Lane 1: Recombinant human PGC protein, C-His, Primary: Anti-PGC (orb1816836) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 40 kDa.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGC) Monoclonal Antibody, Unconjugated (orb1816836) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.