Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]

• Catalog Number: orb1674970
• Category: Antibodies
• Description: Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Goat
• Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This product is an IgG fraction purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.
• Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.
• Immunogen: Goat IgG, whole molecule
• Tested applications: DOT, ELISA, WB
• Application notes: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
• Antibody Type: Secondary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only