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![Peroxidase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]](/images/quality-badge_250x250_1.png){kind=icon}
Peroxidase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]
Catalog Number: orb1675014
| Catalog Number | orb1675014 |
|---|---|
| Category | Antibodies |
| Description | Peroxidase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit] |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Donkey |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG and Donkey Serum. |
| Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Donkey IgG and Donkey Serum. |
| Immunogen | Donkey IgG whole molecule |
| Tested applications | DOT, ELISA, WB |
| Application notes | This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. |
| Antibody Type | Secondary Antibody |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
| Note | For research use only |
