PDCD1/CD279/PD-1 Antibody

• Catalog Number: orb1536020
• Category: Antibodies
• Description: PDCD1/CD279/PD-1 Antibody
• Target: PDCD1 / CD279 / PD-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 16 AA synthetic peptide near the center of human PD-1. The immunogen is located within aa 120-170 of PD-1.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1 µg/ml)
• Application notes: Further information: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL and immunocytochemistry at 10 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 43 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLE
• Note: For research use only

Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 ug/ml.

Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 ug/ml. Green: PD-1 Antibody Blue: DAPI staining

Western blot of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.