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Catalog Number | orb1955389 |
---|---|
Category | Proteins |
Description | PD-L1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.6 kDa and the accession number is Q3U472. |
Tag | C-hFc |
Purity | 98.00% |
MW | 51.6 kDa (predicted); 74.3 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q3U472 |
Expression System | HEK293 Cells |
Biological Origin | Mouse |
Biological Activity | PD-L1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.6 kDa and the accession number is Q3U472. |
Expression Region | A DNA sequence encoding the mouse Cd274 (NP_068693.1) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 19 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of mPD-L1-hFc is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
> 90% as determined by SDS-PAGE | |
51.5 kDa |
98.00% | |
53.3 kDa (predicted) |