PAX5 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499314
• Category: Antibodies
• Description: PAX5 Recombinant Rabbit Monoclonal Antibody
• Target: PAX5
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human PAX5 (250-300aa)
• UniProt ID: Q02548
• MW: 42 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: R6F9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B cell lineage specific activator; B cell lineage
• Research Area: Developmental Biology, Neurogenesis, Neuroscience,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1. Jurkat (Negative control), 2: Ramos, 3: Raji, 4: Daudi, 5: Mouse spleen, 6: Rat, spleen, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 42 kDa, Observed MW: 45 kDa.

Tissue: Human appendix, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal, for orb1499314.

Tissue: Human kidney (Negative tissue), Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: No staining on nucleus.