Parathyroid Hormone Antibody / N Terminal

• Catalog Number: orb534065
• Category: Antibodies
• Description: Parathyroid hormone (PTH), parathormone or parathyrin, is secreted by the chief cells of the parathyroid glands as a polypeptide containing 84 amino acids, yet effective hormone-receptor interaction requires solely the 34-N-terminal amino acids. While PTH acts to increase the concentration of ionic calcium (Ca2+) in the blood, calcitonin, a hormone produced by the parafollicular cells (C cells) of the thyroid gland, acts to decrease ionic calcium concentration. [Wiki]
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A synthetic peptide from the N-terminal region of human Parathyroid hormone was used as the immunogen for the recombinant Parathyroid Hormone antibody.
• UniProt ID: P01270
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant Parathyroid Hormone antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: PRTM1-1R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC testing of FFPE human parathyroid gland stained with recombinant Parathyroid Hormone antibody (clone PRTM1-1R). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.