PALB2 Rabbit Polyclonal Antibody

• Catalog Number: orb11224
• Category: Antibodies
• Description: PALB2 Rabbit Polyclonal Antibody
• Target: PALB2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PALB2 (1101-1186/1186aa)
• UniProt ID: Q86YC2
• RRID: AB_10751809
• MW: 131 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DKFZp667I166; 4732427B05; BC066140; DKFZp686E1054;
• Note: For research use only

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (orb11224) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Independently Validated Antibody, Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (orb11224) at 1:100 from one hour at room temperature followed by conjugation to the secondary antibody Mach2 rabbit HRP-Polymer for 30 minutes at room temperature. Positive Control, the staining is correct.

Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PALB2 Polyclonal Antibody, Unconjugated (orb11224) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.