p53 Tumor Suppressor Protein Antibody

• Catalog Number: orb389251
• Category: Antibodies
• Description: Mouse monoclonal antibody to p53
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BP53-12
• Tested applications: IHC, WB
• Reactivity: Canine, Gallus, Hamster, Human, Monkey
• Isotype: IgG2a
• Immunogen: Recombinant human wild-type p53 protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 53kDa.
• Target: TP53
• Entrez: 7157
• UniProt ID: P04637
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: anti BCC7 antibody, anti FLJ92943 antibody, anti H
• Note: For research use only
• Application notes: p53 Tumor Suppressor Protein
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A431, MCF7, HEK293 Lysate using p53 antibody

Immunohistochemical staining of human Colon carcinoma tissue using p53 antibody