P53 Antibody

• Catalog Number: orb1925686
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1711CT184.18.1
• Tested applications: IHC-P, WB
• Reactivity: Human, Monkey
• Isotype: IgG1,k
• Dilution range: IHC: 1:400, WB: 1:2000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 43653 Da
• Target: This P53 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 1-393 amino acids from human P53.
• UniProt ID: P04637
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cellular tumor antigen p53, Antigen NY-CO-13, Phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human Colon cancer section using Pink1. Diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-P53 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: CCRF-CEM whole cell lysate. Lane 4: COS-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.