OGDH Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172532
• Category: Antibodies
• Description: OGDH Recombinant Mouse Monoclonal Antibody
• Target: OGDH
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG2b/Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: F8B3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: Raji, 2: HepG2, 3: MCF-7, 4: Rat heart, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 116 kDa, Observed MW: 116 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172532.