NTF3 Antibody (C-term)

• Catalog Number: orb1928914
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14406
• Tested applications: FC, WB
• Predicted Reactivity: Other, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 29355 Da
• Target: This NTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-184 amino acids from the C-terminal region of human NTF3.
• UniProt ID: P20783
• NCBI: NP_002518.1, NP_001096124.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-NTF3 Antibody (C-term) at 1:2000 dilution + NTF3 protein at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13. 5 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human brain lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human ovary lysate. Lane 2: Human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.