NR5A2 / LRH1 Antibody

• Catalog Number: orb1248278
• Category: Antibodies
• Description: NR5A2 / LRH1 Antibody
• Target: NR5A2 / LRH1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TDYDRSPFVTSPIS
• UniProt ID: O60587
• MW: Approx 55kDa
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NR5A2, nuclear receptor subfamily 5, group A, memb
• Research Area: Cancer Biology, Epigenetics & Chromatin
• Note: For research use only
• NCBI: NP_995582.1, NP_003813.1

orb1248278 (1 ug/ml) staining of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248278 (0.3 ug/ml) staining of Pig Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.