NFκB-p105 (phospho Ser932) rabbit pAb

• Catalog Number: orb764242
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF kappa B-p105 (phospho-Ser932)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser932. AA range:899-948
• MW: 105
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-NFKB1 antibody, anti-Nuclear factor NF-kappa-
• Note: For research use only

Western Blot analysis of various cells using Phospho-NFκB-p105 (S932) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p105/p50 (Phospho-Ser932) Antibody.

Western blot analysis of lysates from HeLa cells treated with CA + TNF 20ng/ml 10', using NF-kappaB p105/p50 (Phospho-Ser932) Antibody. The lane on the left is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).