N-Cadherin Antibody / CDH2

• Catalog Number: orb2637239
• Category: Antibodies
• Description: N-Cadherin/CDH2/Cadherin-2 acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDH2/6857R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant full-length human CDH2 protein was used as the immunogen for the recombinant N-Cadherin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant N-Cadherin antibody is available for research use only.
• UniProt ID: P19022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant N-Cadherin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of intercalated discs in FFPE human heart tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of human heart tissue lysate using recombinant N-Cadherin antibody (clone CDH2/6857R). Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).