MUC1 Antibody / Mucin-1

• Catalog Number: orb2638112
• Category: Antibodies
• Description: This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MUC1/845
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this EMA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EMA antibody is available for research use only.
• Entrez: 4582
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).

SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.