MUC-4 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1152002
• Category: Antibodies
• Description: MUC-4 Recombinant Rabbit Monoclonal Antibody
• Target: MUC4
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human MUC-4
• UniProt ID: Q99102
• MW: 80 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-200, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: M1F3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Mucin4; Mucin-4; Mucin 4; MUC-4; Ascites sialoglyc
• Research Area: Cancer Research, Defensins, Immune System
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell line: MCF-7, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152002.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MUC-4) Monoclonal Antibody, Unconjugated (orb1152002) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MUC-4) Monoclonal Antibody, Unconjugated (orb1152002) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.