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Catalog Number | orb1931886 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB30652 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:2000, IHC-P: 1:100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 44363 Da |
Target | This Mouse Txnip antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of mouse Txnip. |
UniProt ID | Q8BG60 |
NCBI | NP_076208.2, NP_001009935.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Thioredoxin-interacting protein, Vitamin D3 up-reg Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis on paraffin-embedded Mouse liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Anti-Mouse Txnip Antibody (Center) at 1:2000 dilution + Mouse stomach tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.